Reduced Dietary Protein Induces Changes in the Dental Proteome

Abstract

Experimental studies have demonstrated that nutritional changes during development can result in phenotypic changes to mammalian cheek teeth. This developmental plasticity of tooth morphology is an example of phenotypic plasticity. Because tooth development occurs through complex interactions between manifold processes, there are many potential mechanisms which can contribute to a tooths norm of reaction. Determining the identity of those mechanisms and the relative importance of each of them is one of the main challenges to understanding phenotypic plasticity. Quantitative proteomics combined with experimental studies allow for the identification of potential molecular contributors to a plastic response through quantification of expressed gene products. Here, we present the results of a quantitative proteomics analysis of mature upper first molars in Mus musculus from a controlled feeding experiment. Pregnant and nursing mothers were fed either a low-dietary protein (10%) treatment diet or control (20%) diet. Low-dietary protein was not associated with reduced molar size or skull length. However, expression of tooth-related proteins, immune system proteins, and actin-based myosin proteins were significantly altered in our low-dietary protein proteomics sample. The differential expression of immune proteins along with systematic reduction in actin-based myosin protein expression are novel discoveries for tooth proteomics studies. We propose that studies that aim to elucidate specific mechanisms of molar phenotypic plasticity should prioritize investigations into the relationships between IGF regulation and tooth development and actin-based myosin expression and tooth development. Research HighlightsA low-protein diet during development results in significantly altered protein expression for odontogenetic and osteogenic proteins, immune system proteins, and actin-based myosin proteins within Mus musculus, but does not alter skull length or molar size. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=53 SRC="FIGDIR/small/632248v2_ufig1.gif" ALT="Figure 1"> View larger version (17K): org.highwire.dtl.DTLVardef@2df942org.highwire.dtl.DTLVardef@477dfforg.highwire.dtl.DTLVardef@1a11da0org.highwire.dtl.DTLVardef@a012fc_HPS_FORMAT_FIGEXP M_FIG Graphical Abstract C_FIG