Cell-cycle dependence of bursty gene expression: insights from fitting mechanistic models to single-cell RNA-seq data

Abstract

Bursty gene expression is characterised by two intuitive parameters, burst frequency and burst size, the cell-cycle dependence of which has not been extensively profiled at the transcriptome level. In this study, we estimate the burst parameters per allele in the G1 and G2/M cell-cycle phases for thousands of mouse genes by fitting mechanistic models of gene expression to mRNA count data, obtained by sequencing of single cells whose cell-cycle position has been inferred using a deep-learning method. We find that upon DNA replication, the median burst frequency approximately halves, while the burst size remains mostly unchanged. Genome-wide distributions of the burst parameter ratios between the G2/M and G1 phases are wide, indicating substantial heterogeneity in transcriptional regulation patterns. We also observe a significant negative correlation between the burst frequency and size ratios, suggesting that regulatory processes do not independently control the burst parameters. Finally, we argue that to accurately estimate the burst parameter ratios, mechanistic models must explicitly account for gene copy number variation and extrinsic noise due to the coupling of transcription to cell age within the cell cycle, but corrections for technical noise due to imperfect capture of RNA molecules in sequencing experiments are less critical.