Barriers Broken: Genetic Swamping in Restored Brook Trout Populations

Abstract

Scientists use reintroductions to restore native species to their historical ranges but sometimes can overlook the effects of dispersal on the genetic structure of restored populations. In the Great Smoky Mountains National Park (GRSM), an ambitious reintroduction strategy aims to restore native Brook Trout (Salvelinus fontinalis) populations, which have lost over 75% of their historical habitat within the park. Each restoration requires removing non-native trout and selecting restoration streams with natural barriers to prevent recolonization. Multiple Brook Trout populations are mixed in restoration sites to maximize genetic diversity and to avoid depletion of any single source population. However, a miscommunication during a reintroduction in Anthony Creek, which used three source populations for restoration, inadvertently created an in-situ population genetics study. One segment of the restoration site received fish from all three source populations. However, technicians also unintentionally placed fish from just one source upstream of a natural barrier. Unbeknownst to management, fish from this separate translocation dispersed downstream, potentially altering the desired genetic diversity of the restored population. This study characterizes the genetic changes caused by this unidirectional dispersal. Population genetics theory predicts that such movement leads to genetic swamping. Here, we use genetic and population density data to confirm the directionality of dispersal, estimate the rate of genetic swamping, and assess alternative mitigation strategies. Our results indicate that it is already too late for assisted migration within the restored stream to achieve the intended genetic diversity. Instead introducing additional fish above the second natural barrier would be necessary to equalize the contribution of all three source populations. Understanding the interplay between dispersal behavior and genetic structure is crucial for planning reintroductions and refining conservation strategies for this iconic species.