A Comparison Of Fecal Glucocorticoid Metabolite Concentration And Gut Microbiota Diversity In Bonobos (Pan paniscus)

Abstract

Sex, age, diet, stress, and social environment have all been shown to influence the gut microbiota. In several mammals, including humans, increased stress is related to decreasing gut microbial diversity and may differentially impact specific taxa. Recent evidence from gorillas shows fecal glucocorticoid metabolite concentration (FGMC) did not significantly explain gut microbial diversity, but it was significantly associated with the abundance of the family Anaerolineaceae. These patterns have yet to be examined in other primates, like bonobos (Pan paniscus). We compared FGMC to 16S rRNA amplicons for 201 bonobo fecal samples collected across five months to evaluate the impact of stress, measured with FGMC, on the gut microbiota. Alpha diversity measures (Chaos and Shannons indexes) were not significantly related to FGMC. FGMC explained 0.08% of the variation in beta diversity for Jensen-Shannon and 1.27% for weighted UniFrac but was not significant for unweighted UniFrac. We found that genus SHD-231, a member of the family Anaerolinaceae had a significant positive relationship with FGMC. These results suggest that bonobos are relatively similar to gorillas in alpha diversity and family Anaerolinaceae responses to FGMC, but different from gorillas in beta diversity. Members of the family Anaerolinaceae may be differentially affected by FGMC across great apes. FGMC appears to be context dependent and may be species-specific for alpha and beta diversity but this study provides an example of consistent change in two African apes. Thus, the relationship between physiological stress and the gut microbiome may be difficult to predict, even among closely related species. Graphical Abstract: See PDF Legend: We compared the bonobo gut microbiota to fecal glucocorticoid metabolite concentrations (FGMC). FGMC did not explain alpha diversity, but FGMC explained [~]1.5% of the variation in beta diversity.