Photosystem genes in chloroplast and their interacting partners: A case for molecular adaptation to carnivory in Nepenthaceae.

Abstract

Molecular adaptations are prevalent in carnivorous plants in response to habitat and environmental stress. We used the chloroplast genome and characterized the specific adaptations in the photosystem genes and their interacting partners in Nepenthes khasiana, a carnivorous pitcher plant. When compared with the carnivorous and non-carnivorous groups across Caryophyllales, Lamiales, Poales, Ericales, and Oxalidales, we found Nepenthes-specific changes in psaA, psaB, psaC and psaH. Of these, only a single amino acid change each, G147 in the protein psaA and R40 in the protein ndhD, impacted the three-dimensional structural conformation of the corresponding proteins. Modeling the interaction between the psaA and the ndhD proteins identified group-specific changes between the models between Nepentheceae versus others. The least distance between the structure-impacting residues of psaA and ndhD was 25.9 [A] for Nepenthes and 19.4 [A] for non-Nepenthes models. Given that the chloroplast ndh and photosystem I subunits form a large super-complex with the light-harvesting carrier proteins from the nucleus to mediate cyclic electron transport, our observations may indicate specific adaptations in the cyclic electron transport arm of the photosynthetic machinery in the Nepenthes species.