Organ Scaffold Characterizations Using Intravital Microscopy

Abstract

This study contains intravital microscopy (IVM) data to characterize organ scaffolds created using decellularization. Decellularization creates cell-free collagen-based matrices from native organs, which can be used as scaffolds for regenerative medicine applications. This data set contains in vivo assays evaluating the effectiveness of decellularization and structural and functional integrity of the acellular nephron in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, structural and functional integrities of various decellularized nephron segments, and velocity within the microcirculation were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n=4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). OIF formatted files illustrate IVM measurement processes and are made publicly available in a data repository. Such data is intended to support scientific reproducibility, complement future renal studies, and extend the use of this powerful imaging application to analyze other organ scaffold systems. O_TBL View this table: org.highwire.dtl.DTLVardef@4937b8org.highwire.dtl.DTLVardef@e91b6forg.highwire.dtl.DTLVardef@83eeforg.highwire.dtl.DTLVardef@15be0d4org.highwire.dtl.DTLVardef@105a2e5_HPS_FORMAT_FIGEXP M_TBL C_TBL Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.18703949.