comparison of genetic diversity and phylogenetic structure of 18S gene of Trichomonas vaginalis in Hainan and Xinjiang provinces

Abstract

Trichomonas vaginalis is a kind of flagellate parasite endemic all of the world. study compared the difference of genetic diversity and phylogenetic structure of 18S ribosomal RNA gene of T. vaginalis from Hainan and Xinjiang Provinces, China. 20 samples and 47 samples from Hainan and Xinjiang respectively which confirmed for infection with T.vaginalis. The sequences were aligned using MEGA 11 software and phylogenetic trees were drawn by Neighbor-Joining method. The number of mutation, nucleotide diversity, and haploid diversity were analyzed using Dnaspv5 software. For Hainan samples, the analyses showed 31 polymorphisms, creat different haplotypes with a haplotype diversity of 0.589. Nucleotide diversity and average nucleotide different among T.vaginalis were estimated as 0.00679 and 3.447, respectively.Tajimas D value in Tajiamas neutrality test was -2.48147,which was significant (p<0.001). But for Xinjiang samples, the analyses showed 31 polymorphisms, creat different haplotypes with a haplotype diversity of 0.731. Nucleotide diversity and average nucleotide different among T.vaginalis were estimated as 0.00497 and 2.50435, respectively. Tajimas D value in Tajiamas neutrality test was -2.26284, which was significant (p<0.01). For all the sequence in this study, all the sequences form one phylogenetic tree, some of the sequence from two provinces showed a certain aggregating phenomenon. The differences between the samples from two province maybe for the local environment, ethnic, sample number and so on, it need further study for T.vaginalis.