Benchmarking fungal species classification using Oxford Nanopore Technologies long-read ITS metabarcodes
Abigail Graetz, Jinghang Feng, Alex Ringeri, Austin Bird, Duong Vu, Camille Truong, Benjamin Schwessinger
This study benchmarks species classification of fungal ITS metabarcodes using ONT long-read sequencing, showing that threshold-based and machine learning classifiers outperform others in accuracy and consistency.
Fungal Genetics and Biology · Oct 25, 2025Peer-reviewedMolecular Biology