Surveying armadillo and bat trypanosomes by DNA metabarcoding with Oxford Nanopore Technologies sequencing: the importance of fine-tuning parameters to identify mixed infections
Christian Miguel Pinto, Manuel Calvopiña, Sofía Ocaña-Mayorga, Daniel Romero-Álvarez, Carlos Bastidas‐Caldes, Pamela Lojan-Cueva, Daniela Reyes-Barriga, Alejandra Bedoya-Jaramillo, Víctor Romero, Nicté Ordóñez‐Garza, Andrea Au Hing, Mónica I. Páez‐Vacas, Julio C. Carrión–Olmedo, Ricardo Patiño, Pablo Jarrín–V
This study uses DNA metabarcoding to analyze Trypanosoma infections in armadillos and bats, finding that strict bioinformatic filtering reduces apparent coinfections, supporting competitive exclusion.
bioRxiv · Aug 7, 2026 · PreprintMicrobiology