Stepwise Proteinase K Digestion Improves Genomic DNA Yield and Purity from Goat Semen: A Comparative Pilot Study of Five Extraction Protocols
Sharadindu Shil, Ankur Singha, Soumita Paul, Ashish Khatua, Shankari Prasad Dutta, Tarpan Mondal, Jayanta Chowdhury, Ayan Mukherjee, Anusuya Debnath, Ajoy Mandal
This study compares five DNA extraction protocols from goat semen, finding that Modified Protocol 2 provides the highest DNA yield, purity, and PCR success.
bioRxiv · Oct 17, 2026 · PreprintMolecular Biology