Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation
M. Gonzalez Pisfil, I. Nadelson, B. Bergner, S. Rottmeier, A. W. Thomae, S. Dietzel
The paper demonstrates a five-color STED microscopy approach using fluorescence lifetime phasor separation with a single depletion laser, achieving higher color resolution than traditional methods.
bioRxiv · Apr 13, 2022Cell Biology