Uncovering Functional Gene Regulatory Networks in Bulk and Single-Cell Data through Robust Transcription Factor Activity Estimation and Model-Guided Experimental Validation
Alireza Fotuhi Siahpirani, Sunnie Grace McCalla, Saptarshi Pyne, Caleb M. Dillingham, Rupa Sridharan, Sushmita Roy
The paper introduces MERLIN+P+TFA to robustly estimate transcription factor activity and infer gene regulatory networks from bulk and single-cell data, showing improved accuracy in yeast and mammalian systems.
bioRxiv · Jun 9, 2025 · PreprintBioinformatics