Systematically investigating the key features of the nuclease deactivated Cpf1 for tunable multiplex genetic regulation
Chensi Miao, Huiwei Zhao, Long Qian, Chunbo Lou
This study identifies key features of nuclease-deactivated Cpf1 for multiplex gene regulation, including novel PAM patterns and improved gene repression through crRNA modifications.
bioRxiv · Apr 9, 2018 · PreprintSynthetic Biology