intI1 primer selection for class 1 integron integrase gene and transcript quantification - validation and application for monitoring intl1 gene abundance within septic tanks in Thailand
Valentine Okonkwo, Fabien Cholet, Umer Zeeshan Ijaz, Thammarat Koottatep, Tatchai Pussayanavin, Chongrak Polpraset, William T. Sloan, Stephanie Connelly, Cindy J. Smith
The paper validates and applies intI1 primer sets for quantifying gene abundance in septic tanks, finding no significant difference between primer sets but highlighting the importance of primer choice for accurate environmental monitoring.
Applied and Environmental Microbiology · Oct 24, 2023Peer-reviewedMolecular Biology