Comparative Performance of Portable DNA Extraction Protocols and Bioinformatics Workflows for Rapid Detection of Pathogens and Antimicrobial Resistance Using Oxford Nanopore Sequencing.
Louis Kyei-Tuffuor, Samuel Kekeli Agordzo, Angela Krobea Asante, Davina Serwaa Boateng, Winifred Nana Serwaa Adjei, Venus Nana Boakyewaa Frimpong, Rita Nartey, Francis Agyemang-Yeboah, Rea Maja Kobialka, Ahmed Abd El Wahed, Uwe Truyen, Yaw Ampem Amoako, Richard Odame Phillips, Michael Frimpong
This study compares DNA extraction protocols and bioinformatics workflows for Oxford Nanopore sequencing of bacterial pathogens, finding that extraction method significantly impacts workflow success and genomic resolution.
bioRxiv · Feb 26, 2026 · PreprintGenomics