The meaning of the Michaelis-Menten constant: Km describes a steady-state.
E. I. Canela, G. Navarro, J. L. Beltran, R. Franco
The paper clarifies that the Michaelis-Menten constant (Km) defines the steady state in enzyme kinetics, not the substrate dissociation constant (Kd), and emphasizes the importance of the association rate (k1) in physiological contexts.
bioRxiv · May 15, 2019 · PreprintBiochemistry