CYP154C5 regioselectivity in steroid hydroxylation explored by substrate and protein engineering
P. Bracco, H. J. Wijma, B. Nicolai, J. A. Rodriguez Buitrago, T. Kluenemann, A. Vila, P. Schrepfer, W. Blankenfeldt, D. B. Janssen, A. Schallmey
The paper explores how altering CYP154C5's regioselectivity in steroid hydroxylation can be achieved through protein and substrate engineering, revealing structural and dynamic changes in the active site.
bioRxiv · Mar 4, 2020Biochemistry