Is Protein Quantification and Physical Normalization Always Necessary in Proteomics?
Alex Zelter, Michael E. Riffle, Gennifer E. Merrihew, Batool Mutawe, Aaron J. Maurais, Han-yin Yang, Jamie L. Inman, S Celniker, Jian-Hua Mao, Kenneth H. Wan, Antoine M. Snijders, Christine C. Wu, Michael J. MacCoss
This study investigates whether protein quantification and physical normalization are necessary in proteomics by comparing normalized and unnormalized sample inputs, showing that computational normalization can reduce variability in some cases.
bioRxiv · Feb 28, 2026 · PreprintBiochemistry