Identifying multiple kinetic populations of DNA binding proteins in live cells using single-molecule fluorescence imaging
H. N. Ho, D. Zalami, J. Köhler, A. M. van Oijen, H. Ghodke
This study analyzes the limitations of time-lapse single-molecule fluorescence imaging in measuring kinetic sub-populations of DNA-binding proteins and provides guidelines for reliable data collection and interpretation.
bioRxiv · Jul 25, 2019Biophysics