Dark-based Optical Sectioning assists Background Removal in Fluorescence Microscopy
Ruijie Cao, Yaning Li, Yao Zhou, Meiqi Li, Fangrui Lin, Wenyi Wang, Guoxun Zhang, Gang Wang, Boya Jin, Wei Ren, Yu Sun, Zhifeng Zhao, Wei Zhang, Jing Sun, Yiwei Hou, , Peng Xi
This paper introduces a method to remove defocus backgrounds in fluorescence microscopy using dark channels and dual frequency separation, significantly improving image quality.
Nature Methods · May 12, 2025Peer-reviewedBioinformatics