experimental assay

SPACE-Tag Enables High-Throughput Spatial Chromatin Profiling With CUT&Tag

The paper introduces SPACE-Tag, a spatial CUT&Tag method for chromatin profiling, and applies it to the mouse brain to reveal age-related chromatin and transcriptome changes.

Microbially conjugated bile acids differentially affect the lifecycle and pathogenesis of Clostridioides difficile

The paper synthesizes and tests microbially conjugated bile acids (MCBAs) to determine their effects on Clostridioides difficile lifecycle stages, revealing amino acid-dependent inhibitory or promoting effects.

The methionine cycle sustains the mesenchymal phenotype of tumor cells and drives PDA progression

This study identifies the methionine cycle as a driver of mesenchymal traits in PDA, showing that targeting SAHH reduces metastasis and improves survival.

Proteome-wide discovery of novel diagnostic antigens for coccidioidomycosis

This study identifies novel diagnostic antigens for coccidioidomycosis using high-dimensional peptide serology, showing improved accuracy in distinguishing infected individuals from controls.

Citrobacter rodentium infection disrupts colonic mucus barrier function via ablation of intercrypt goblet cells

This study shows that Citrobacter rodentium infection disrupts the colonic mucus barrier by ablating intercrypt goblet cells, leading to impaired barrier integrity and enhanced pathogen colonization.

Dual kinase inhibition provides early clues to restoration of neuroimmune homeostasis in an AAV-haSyn model of Parkinsons disease

This study shows that dual inhibition of MLK3 and LRRK2 with URMC-099 reduces PD pathology and motor deficits in an AAV-haSyn rat model.

Identification and characterization of new P. aeruginosa phages using a surface receptor platform

The paper introduces a SURFAR platform to identify and characterize new P. aeruginosa phages by targeting specific receptors, isolating two novel phages with distinct host ranges.

Fatty Acid Synthase Binds Cellular mRNAs and its Depletion Reduces Translational Efficiency

This study identifies FASN's RNA binding sites and shows its depletion reduces translational efficiency of associated mRNAs.

Cryo-EM structures of an Acinetobacter baumannii podovirus reveal a canonical capsid maturation

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

Magnetic organelle gene clusters enable bacterial growth and ATP homeostasis under oxidizing conditions

This study shows that gene clusters for magnetite formation in bacteria confer resistance to hydrogen peroxide, supporting an early origin of magnetotactic bacteria before Earth's oxygenation.

Shikonin alleviates bone cancer pain through suppression of spinal IGF2-associated p38 MAPK signaling

This study shows that intrathecal Shikonin reduces bone cancer pain in rats by suppressing spinal IGF2-associated p38 MAPK signaling, improving pain-related behaviors and reducing inflammation.

Epidermal Langerhans Cells Act as Neuroimmune Regulators of Pain and Itch

This study identifies Langerhans cells as immune-derived amplifiers of cutaneous sensory signaling and identifies semaphorin 7A as a mediator of LC-induced itch potentiation.

Broad Cognitive and Motivational Enhancement through M4-Selective Muscarinic Modulation

This study evaluates a M4-selective PAM in nonhuman primates, showing broad cognitive and motivational improvements without adverse side effects.

Massive inter-animal horizontal gene transfer contributes to stress tolerance in the fungus gnat Bradysia coprophila

This study identifies extensive horizontal gene transfer in the fungus gnat Bradysia coprophila, showing these genes contribute to stress tolerance and ecological adaptation.

Preserving Nanotherapy-Induced Allogeneic Murine Islet Graft Tolerance During Distal Methicillin-Resistant Staphylococcus aureus Skin Infection

This study shows that a nanotherapy (rPS) that induces islet graft tolerance can be compromised by MRSA infection, but combining it with methylprednisolone improves survival.

Network analysis of mouse brain proteomes resolves molecular and cellular endophenotypes of aging and Aβ pathology

This study uses proteomics to identify aging and Aβ-related molecular changes in mouse brains and finds they are conserved in human AD proteomes.

From Fill to Freeze: Redesigning homeRNA to Enable Robust Sampling and Scalable Downstream Processing Workflows for Remote Blood Transcriptomics

This paper validates the redesigned homeRNA platform's robustness to remote study stresses, showing it maintains RNA quality under varied conditions and storage times.

A novel KIR3DL2-directed CAR T-cell therapy for cutaneous and systemic T-cell lymphomas

This paper develops a KIR3DL2-directed CAR T-cell therapy for T-cell lymphomas, showing preclinical efficacy and safety in xenograft models.

ER stress-associated IRE1α signalling links impaired QPRT-dependent NAD⁺ biosynthesis to reduced oocyte competence

This study links ER stress and impaired NAD biosynthesis via QPRT to reduced oocyte quality and shows that NMN supplementation improves oocyte function and early embryonic development.

Mutant Isocitrate Dehydrogenase 1 Alters Extracellular Vesicle Secretion and Cargo Composition in Cellular Models

This study shows mutant IDH1 increases sEV secretion and alters their cargo composition in glioma and chondrosarcoma cells, with D2HG packaged into sEVs and cell-type-specific cargo remodeling.

The biochemical composition of coronavirus Nucleocapsid proteins drives the bicistronic properties of their sub-genomic mRNAs

The paper identifies conserved overlapping accessory ORFs in coronavirus N genes and shows that nucleotide and codon biases drive their formation.

CD300f is required for efficient lipid handling during cuprizone-induced demyelination

This study shows CD300f is essential for microglial lipid handling and remyelination during demyelination, with deficits in lipid metabolism and motor coordination in its absence.

Motivation-dependent communication between cortex and brainstem

The paper investigates how motivational context affects cortico-medullary communication during different types of licking behavior in mice.

Endothelial protein C receptor is an entry receptor for Langya virus

This study identifies the endothelial protein C receptor (EPCR) as the entry receptor for Langya virus and reveals the molecular basis of its interaction with the viral attachment glycoprotein.

Organotchi: an open-source tool for the automated segmentation, morphological characterization, and longitudinal tracking of organoids

Organotchi automates segmentation and morphological analysis of neural organoids, quantifying features and tracking changes over time to improve quality assessment and downstream analysis.

Neurons entrain and shape an intrinsic arteriole rhythm that distributes blood across the cortical mantle

The paper shows neurons entrain an intrinsic arteriole rhythm, shaping blood distribution and vascular responses through frequency-dependent mechanisms.

Asymmetric division and polar growth define a non-canonical cell cycle in Bradyrhizobium.

This study describes the asymmetric cell division and polar growth in Bradyrhizobium, revealing a unique cell cycle program with distinct nucleoid dynamics and chromosome organization.

Global repression of mesenchymal genes in epithelial cells by the histone demethylase LSD1

This study shows LSD1 represses mesenchymal genes in epithelial cells, maintaining epithelial identity, and challenges the therapeutic use of LSD1 inhibition for EMT control.

Satellite cell glucocorticoid receptor restrains myogenic commitment without mediating the effects of therapeutic glucocorticoids on muscle repair

This study shows that satellite cell glucocorticoid receptor (GR) maintains quiescence but does not mediate therapeutic GC effects on muscle repair, highlighting the importance of treatment timing and context.

Seed Amplification Assay-Based Detection of Alpha-Synuclein Aggregates Outperforms Immunohistochemical Analysis in the Intestinal Mucosal Biopsies of Parkinson's Disease and Multiple System Atrophy Participants

This study compares a seed amplification assay for alpha-synuclein aggregates with immunohistochemistry in intestinal biopsies of Parkinson's disease and multiple system atrophy patients, finding the assay detects aggregates more frequently and with higher specificity.