DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing
Peng Ni, Fan Nie, Zeyu Zhong, Jinrui Xu, Neng Huang, Jun Zhang, Haochen Zhao, You Feng Zou, Yuanfeng Huang, Jinchen Li, Chuan‐Le Xiao, Feng Luo, Jianxin Wang
The paper introduces ccsmeth, a deep-learning method for detecting DNA 5mCpGs using PacBio CCS, achieving high accuracy and AUC, and presents ccsmethphase for haplotype-aware methylation detection.
Nature Communications · Jul 8, 2023Peer-reviewedBioinformatics