Perfect 21-nucleotide matches to beneficial fungi are common in canonical antifungal dsRNA targets: an in-silico off-target hazard screen for spray-induced gene silencing
Muhammad Wasif, Gábor Tarcali, Moman Khan, Huma Saleem, Muhammad Abubakkar Azmat
The paper screens dsRNA constructs for off-target effects on beneficial fungi and finds many have perfect 21-nt matches, highlighting a regulatory gap.
bioRxiv · Sep 28, 2026 · PreprintBioinformatics