Ecological Observations Based on Functional Gene Sequencing Are Sensitive to the Amplicon Processing Method
Fabien Cholet, Agata Lisik, Hélène Agogué, Umer Zeeshan Ijaz, Philippe Pineau, Nicolas Lachaussée, Cindy J. Smith
This paper compares OTUs and ASVs methods for analyzing functional gene sequences in microbial communities, showing ASVs provide more consistent results for certain genes.
mSphere · Aug 8, 2022Peer-reviewedBioinformatics