Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.
D. N. Larsen, J. Z. Kaczmarek, Y. Palarasah, J. H. Graversen, P. Hojrup
This study presents a method using Fenton chemistry and HRPF to map epitopes on SARS-CoV-2 RBDs, emphasizing the importance of negative antibody controls and technical replicates to reduce false positives.
bioRxiv · Oct 27, 2023Biochemistry