Super-multiplexed fluorescence microscopy via photostability contrast
A. Orth, R. N. Ghosh, E. R. Wilson, T. Doughney, H. Brown, P. Reineck, J. Thompson, B. C. Gibson
This paper introduces a method to increase fluorescence microscopy multiplexing by using photostability differences and a novel unmixing algorithm, enabling up to three labels per spectral channel.
bioRxiv · Feb 2, 2018Bioengineering