High-Throughput Single-Cell Spectroscopy Using Phasor Analysis of Spectral Flow Cytometry
Bruno Pannunzio, Paula Cespedes, Marcela Díaz, Daniela Ali, A. Fernández Rial, Leonel S. Malacrida
This paper introduces phSFC, a method using spectral flow cytometry and phasor analysis to analyze membrane properties in single cells, showing it matches hyperspectral microscopy results.
bioRxiv · Mar 2, 2026 · PreprintBiophysics