Quantifying Uncertainty in Phasor-Based Time-Domain Fluorescence Lifetime Imaging Microscopy
Qinyi Chen, Jongchan Park, Shuqi Mu, Liang S. Gao
This study develops a theoretical uncertainty model for phasor-based FLIM to quantify noise effects on lifetime estimation and fluorophore unmixing, validated with simulations and experiments.
Biomedical Optics Express · Jun 16, 2025Peer-reviewedBioinformatics