Taxonomic classification cost tracks neither sequencing depth nor community richness at single-sample scale: a measured resource protocol for 16S rRNA amplicon pipelines
Sam Victor, Siddharth Sadasivam, S Yuvaraj, R. Subha, Shravan Aarya P, Gugan Satheesh
The paper measures resource usage of 16S rRNA pipeline stages, finding denoising scales with sequencing depth while classification has a fixed cost.
bioRxiv · Sep 1, 2026 · PreprintBioinformatics