Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions
H. J. Ellis, M. Chan, B. Selvam, E. Walter, C. A. Devlin, S. K. Szymanski, L. K. Henry, D. Shukla, E. Procko
This study uses deep mutagenesis to identify mutations enhancing APP+ import by SERT, revealing conformational dynamics and mutationally tolerant regions along the permeation pathway.
bioRxiv · Apr 19, 2021 · PreprintBiochemistry