Enhancing the cell-free expression of native membrane proteins by in-silico optimization of the coding sequence - an experimental study of the human voltage-dependent anion channel
Sonja Zayni, Samar Damiati, Susana Moreno‐Flores, Fabian Amman, Ivo L. Hofacker, David Jin, Eva-Kathrin Ehmoser
This study optimizes the coding sequence of the human VDAC membrane protein to enhance cell-free expression by analyzing translation initiation factors and developing a scoring function for expression prediction.
Membranes · Sep 28, 2021Peer-reviewedMolecular Biology