Transition State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase
B. van Loo, R. Berry, U. Boonyuen, M. F. Mohamed, M. Golicnik, A. C. Hengge, F. Hollfelder
This study investigates the mechanism of sulfate and phosphate monoester hydrolysis by Pseudomonas aeruginosa arylsulfatase using LFERs and KIEs, revealing key roles for active site residues in leaving group stabilization and mechanism shifts.
bioRxiv · May 21, 2018Biochemistry