Selection of internal references for transcriptomics and RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell lines
Y.-H. Gu, X.-W. Cui, J.-Y. Ren, M.-M. Long, W. Wang, C.-J. Wei, R. Aimaier, Y.-H. Li, M.-H. Chung, B. Gu, Q.-F. Li, Z.-C. Wang
This study identifies optimal internal reference genes for transcriptomics and RT-qPCR in NF1-related Schwann cell lines, finding that GAPDH is unstable and recommending PPIA/TBP for malignant lines and PPIA/RPLP0 for benign lines.
bioRxiv · Oct 22, 2020Cancer Biology