Accurate and Fast Protein Acylation Identification by Eliminating Position Effects of Cyclic Immonium Ions with Stepped HCD
Zhao-Yu Zhu, Peng-Zhi Mao, Ching Tarn, Yong Cao
This study identifies a position effect influencing cyclic immonium ion generation in lysine acylations and introduces a stepped HCD strategy to improve PTM detection.
bioRxiv · Jul 3, 2025 · PreprintBiochemistry