Understanding and mitigating some limitations of Illumina (C) MiSeq for environmental sequencing of fungi.
Daniel Churchill Thomas, Roo Vandegrift, Graham Bailes, Bitty A. Roy
The paper identifies and addresses technical limitations of the Illumina MiSeq platform in fungal ITS-amplicon metabarcoding, including read abundance bias and tag-switching issues.
bioRxiv · Sep 10, 2017 · PreprintBioinformatics