Benchmarking full-length ITS metabarcoding across Illumina 2x500, PacBio, and Oxford Nanopore sequencing using mock and soil communities
Leho Tedersoo, Marko Prous, Meirong Chen, Sten Anslan, Irja Saar, Benjamin Dubois, Vladimir S. Mikryukov
The paper benchmarks full-length ITS metabarcoding across Illumina, PacBio, and Oxford Nanopore platforms using mock and soil communities, finding PacBio has the lowest error rate and recommends standard pipelines for biodiversity assessments.
bioRxiv · May 21, 2026 · PreprintBioinformatics