Comparison of real-time quantitative Polymerase Chain Reaction (PCR) and digital droplet PCR for quantification of hormone membrane receptor FSHR, GPER and LHCGR transcripts in human primary granulosa lutein cells
Samantha Sperduti, Clara Lazzaretti, Elia Paradiso, Claudia Anzivino, Maria Teresa Villani, Gaetano De Feo, Manuela Simoni, Livio Casarini
This study compares qPCR and ddPCR for quantifying hormone receptor transcripts in human granulosa cells, finding ddPCR more accurate for low-expression genes.
Gene Reports · May 8, 2021Peer-reviewedGenetics