Methodological pitfalls in plant pangenome gene family identification may lead to biased evolutionary inferences
Shuzhen Liu, Wei Zhang, Pei Yu
The paper shows that using only sequence similarity for gene family clustering in pangenomes can lead to biased evolutionary inferences and recommends a two-step approach combining graph-based orthogroup inference with sequence similarity refinement.
bioRxiv · May 18, 2026 · PreprintGenomics