Differences in molecular sampling and data processing explain variation among single-cell and single-nucleus RNA-seq experiments
John Chamberlin, Younghee Lee, Gábor Marth, Aaron R. Quinlan
The paper reanalyzes RNA-seq data to show pre-mRNA levels vary across cell types, affecting gene length bias and normalization methods, and highlights the impact of bioinformatic processing over assay choice.
Genome Research · Feb 1, 2024Peer-reviewedBioinformatics