Coverslip Hypoxia High-Content Screening (CH-HCS): A 2D Imaging Platform for Spatially Resolved Analysis of CAR-T Function Under Oxygen Gradients
JPZ Murarolli, Giovana Beatriz Capuz Beatriz Capuz, Maria Luiza Arrojo, Amanda Mizukami, Sâmia Rigotto Caruso, Maristela Delgado Orellana, Rodrigo Alexandre Panepucci
The paper introduces CH-HCS, a 2D imaging platform that enables spatially resolved analysis of CAR-T cell function under controlled oxygen gradients, revealing hypoxia's impact on cytotoxicity and motility.
bioRxiv · Feb 2, 2026 · PreprintCancer Biology