Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase

Amber N. Hafeez; Laëtitia Chartrain; Cong Feng; Florence Cambon; Martha Clarke; Simon Griffiths; Şadiye Hayta; Mei Jiang; Beat Keller; Rachel Kirby; Markus C. Kolodziej; Oliver R. Powell; Mark A. Smedley; Burkhard Steuernagel; Wenfei Xian; Luzie U. Wingen; Shifeng Cheng; Cyrille Saintenac; Brande B. H. Wulff; James K. M. Brown

doi:10.1101/2023.09.11.557217

bioRxivSep 11, 2023GeneticsPreprint

Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase

Amber N. Hafeez iD, Laëtitia Chartrain, Cong Feng iD, Florence Cambon, Martha Clarke, Simon Griffiths iD, Şadiye Hayta iD, Mei Jiang iD, Beat Keller iD, Rachel Kirby iD, Markus C. Kolodziej iD, Oliver R. Powell iD, Mark A. Smedley iD, Burkhard Steuernagel iD, Wenfei Xian iD, Luzie U. Wingen iD, Shifeng Cheng iD, Cyrille Saintenac iD, Brande B. H. Wulff iD, James K. M. Brown iD

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Abstract

Septoria tritici blotch (STB), caused by the Dothideomycete fungus Zymoseptoria tritici, is of one of the most damaging diseases of bread wheat (Triticum aestivum)1 and the target of costly fungicide applications2. In line with the fungus apoplastic lifestyle, STB resistance genes isolated to date encode receptor-like kinases (RLKs) including a wall-associated kinase (Stb6) and a cysteine-rich kinase (Stb16q)3,4. Here, we used genome-wide association studies (GWAS) on a panel of 300 whole-genome shotgun-sequenced diverse wheat landraces (WatSeq consortium) to identify a 99 kb region containing six candidates for the Stb15 resistance gene. Mutagenesis and transgenesis confirmed a gene encoding an intronless G-type lectin RLK (LecRK) as Stb15. The characterisation of Stb15 exemplifies the unexpected diversity of RLKs conferring Z. tritici resistance in wheat.

Citations: 11

Wheat and Barley Genetics and Pathology

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