Hippocampal sequences represent working memory and implicit timing

Abstract

Working memory (WM) and timing are considered distinct cognitive functions, yet the neural signatures underlying both can be similar. To address the hypothesis that WM and timing may be multiplexed we developed a novel rodent task where 1st odor identity predicts the delay duration. We found that WM performance decreased when delay expectations were violated. Performance was worse for unexpected long delays than for unexpected short delays, suggesting that WM may be tuned to expire in a delay-dependent manner. Calcium imaging of dorsal CA1 neurons revealed odor-specific sequential activity tiling the short and long delays. Neural sequence structure also reflected expectation of the timing of the 2nd odor--i.e., of the expected delay. Consistent with the hypothesis that WM and timing may be multiplexed, our findings suggest that neural sequences in dorsal CA1 may encode cues and cue-specific elapsed time during the delay period of a WM task.