Identifying the Fusarium species involved in foot rot disease of faba beans in the UK using a combined molecular and microbiological approach

Abstract

Foot rot is a devastating disease of faba bean crops globally, including in the United Kingdom, the worlds third largest producer. To identify the causal agents, we have sampled foot rot-affected plants and soils from faba bean crops across England. We isolated organisms associated with foot rot disease in culture and assessed pathogenicity in vivo to evaluate the infectivity of the isolates on faba bean. We identified the pathogenic isolates using DNA barcoding of the Internal Transcribed Spacer (ITS) and Translation Elongation Factor one (TEF1 ) molecular markers. A total of 113 clonal isolates were obtained from infected plants and soil samples across England. Of these, 60 were pathogenic, inducing mild to severe symptoms on faba bean. Sequencing of the ITS and TEF1 loci and comparison against sequence databases (Genbank and Fusarium_ID) enabled the identification of pathogenic isolates, in decreasing order of frequency, as Fusarium oxysporum (26.6 %), F. vanettenii (25%), F. redolens (15 %), F. solani (11.6%), F. culmorum (8.3 %), F. avenaceum (6.7 %), F. equiseti (1.7 %), F. clavum (1.7 %), Clonostachys rosea (1.7%) and Alternaria alternata (1.7%). F. oxysporum, F. redolens and F. avenaceum induced the most severe symptoms, whilst F. solani induced the least severe symptoms. Determining the most prevalent causal agents of foot rot in UK faba beans will facilitate targeted disease monitoring and intervention for enhanced productivity.