Dysregulation of pupal diapause in hybrid progenies, introgression and species delimitation within and beyond the Old World Swallowtail (Papilio machaon Linnaeus) butterfly complex

Abstract

In search of recurrent patterns of postzygotic hybrid incompatibility, we investigated the Holarctic Old World Swallowtail (Papilio machaon Linnaeus) butterfly complex, whose members can easily be crossed in the laboratory. How many species this model system comprises remains unclear, as taxa with highly distinctive larvae but uncertain status come into contact with authentic P. machaon subspecies in Southern parts of the Palearctic region. By determining mitochondrial and ITS2 haplotypes within and away from contact zones, we found that these neighboring populations do exchange genes, as expected from F1 hybrids being generally fertile in our laboratory crosses. Nevertheless, recurrent instances of dysregulation of diapause were uncovered in hybrid progenies. In keeping with Haldanes Rule, pupae of the heterogametic (female) sex were either unable to enter diapause or, in reciprocal crosses, unable to resume development after having initiated diapause, whereas F1 males experienced normal, photoperiod-regulated diapause, but passed on abnormal diapause regulation to part of their female offspring when backcrossed. Comparing male and female pupal weights in hybrid progenies provides estimates of parental incompatibility that allow to rank taxa and predict quantitatively the outcome of additional crosses, as expected if the same regulatory system were involved. A survey of the entomological literature confirms that diapausing pupae that cannot resume development ( perpetual nymphs) are a recurrent feature of interspecific lepidopteran crosses. Moreover, of the two parent species of a perpetual nymph, the paternal one generally has fewer broods per year. These observations are discussed in the light of models of the speciation process.