Resolving host and species boundaries for perithecia-producing nectriaceous fungi across the central Appalachian Mountains

Abstract

The Nectriaceae contains numerous canker pathogens. Due to scarcity of ascomata on many hosts, comprehensive surveys are lacking. Here we characterize the diversity of perithecia-producing nectriaceous fungi across the central Appalachians. Ten species from twelve hosts were recovered including a novel Corinectria sp. from Picea rubens. Neonectria ditissima and N. faginata were most abundant and associated with Fagus grandifolia with beech bark disease (BBD). N. ditissima was also recovered from additional cankered hardwoods, including previously unreported Acer spicatum, Ilex mucronata, and Sorbus americana. Cross-pathogenicity inoculations of N. ditissima confirmed susceptibility of Acer and Betula spp. Neonectria magnoliae was recovered from cankered Liriodendron tulipifera and Magnolia fraseri and pathogenicity on L. tulipifera was confirmed. Fusarium babinda was consistently recovered from beech with BBD, although its role remains unclear. This survey provides a contemporary snapshot of Nectriaceae diversity across the Appalachian Mountains. The following nomenclatural changes are proposed: Neonectria magnoliae comb. nov.