Phenotypic and omics analyses of the Sordariomycetes Marquandomyces marquandii and Albophoma yamanashiensis isolated from estuarine sediments.

Abstract

Marine environments harbor a vast diversity of microorganisms, which have developed multiple strategies to adapt to challenging conditions and represent a valuable source for new products such as pigments, enzymes and bioactive compounds. From all microorganisms inhabiting marine environments, fungi have been the least studied, despite their ubiquitous presence and great biotechnological potential. Here, we focused on the isolation and characterization of filamentous fungi from marine sediment samples, which were collected along the Basque coast in Spain. Through phenotypic characterization, we identified isolates potentially able to produce secondary metabolites or grow on minimal culture medium supplemented with recalcitrant algal polysaccharides. Based on this screening, two Sordariomycetes isolates were selected for further analyses through genome sequencing and omics techniques: 1) a Marquandomyces marquandii strain able to stain the culture medium in yellow, indicative of secretion of pigments and secondary metabolites and 2) an Albophoma yamanashiensis strain able to grow in minimal culture medium supplemented with the recalcitrant algal polysaccharide fucoidan. Fungal co-culture experiments suggested an inhibitory effect of the secretome of M. marquandii on fungal growth. Under culture conditions inducing pigment secretion, a set of secondary metabolite gene clusters were differentially expressed, as analysed by RNA-seq. On the other hand, transcriptomic and proteomic experiments on A. yamanashiensis unveiled the enzymatic activities expressed in response to the presence of fucoidan. Overall, our results indicate that the isolated marine fungal strains could serve as a source of new enzymatic activities and secondary metabolites.