Osmoregulation affects elimination of microplastics in fish in freshwater and marine environments

Abstract

In recent decades, microplastics (MPs) have emerged as one of the biggest environmental challenges in aquatic environments. Ingestion and toxicity of MPs in seawater (SW) and freshwater (FW) fish have been studied extensively both in field and laboratory settings. However, the basic mechanism of how fish deal with MPs in SW and FW remains unclear, although physiological conditions of fish differ significantly in the two environments. In this study, using Javanese medaka (Oryzias javanicus), a euryhaline fish which adapts readily to both SW and FW, we investigated elimination of MPs in fish in SW and FW environments. We exposed O. javanicus larvae (21 days post-hatching) to 0.25 mg/L of fluorescent polystyrene microspheres (1 {micro}m) for 24 h and then conducted an elimination test for up to 5 days. The results showed that the gut retention time (GRT) of MPs is longer in FW than in SW, indicating that MP elimination occurs more quickly in SW than in FW. However, despite shorter GRTs, higher numbers of MPs tended to be retained for a longer period in SW larvae than FW larvae. Subsequently, using a fluorescent marker, gastrointestinal fluid was found to move more rapidly in the SW group. This finding indicates that water drinking accelerates gastrointestinal fluid movement, which moves MPs through the gut in SW larvae. Beside the difference in physiological conditions, MP elimination was faster when food was available, suggesting that feeding also affects MP elimination in fish. Internal factors such as body size and intestine length were also examined, but indicated no significant difference between SW and FW. Therefore, osmoregulation and feeding both influence MP elimination in fish.