Glutamatergic neuron types in the amygdala of the urodele amphibian Pleurodeles waltl

Abstract

The amygdala is a complex brain structure in the vertebrate telencephalon, essential for regulating social behaviors, emotions and (social) cognition. In contrast to the vast majority of neuron types described in the many nuclei of the mammalian amygdala, little is known about the neuronal diversity in non-mammals, making reconstruction of its evolution particularly difficult. Here, we characterize glutamatergic neuron types in the amygdala of the salamander Pleurodeles waltl. Our single-cell RNA sequencing data indicate the existence of at least ten distinct types and subtypes of glutamatergic neurons in the salamander amygdala. In situ hybridization for marker genes indicates that these neuron types are located in three major subdivisions: the lateral amygdala, the medial amygdala, and a newly-defined area demarcated by high expression of the transcription factor Sim1. The gene expression profiles of these neuron types suggest similarities with specific neuron types in the sauropsid and mammalian amygdala, and in particular the evolutionary conservation of Sim1-expressing amygdalar neurons in tetrapods. Taken together, our results reveal a surprising diversity of glutamatergic neuron types in the amygdala of salamanders, despite the anatomical simplicity of their brain.