Hyperhomocysteinemia does not increase the risk of intracerebral hemorrhage in hypertensive mice

Abstract

ObjectiveHyperhomocysteinemia (HHcy) affects approximately 75% of the population in China, and there is currently controversy regarding whether HHcy increases the risk of hemorrhagic stroke. This study aims to investigate the effects of high homocysteine (Hcy) levels on cerebral hemorrhage in hypertensive mice by administering homocysteine to them. MethodsMale C57BL/6 mice at 8 months of age were used in the experiment. The study was divided into two groups: the Hcy + AngII + L - NAME group and the AngII + L - NAME group. Magnetic resonance imaging (MRI) was performed when the mice exhibited signs of cerebral hemorrhage.After the hemorrhage, anesthesia was induced to euthanize the animals, and then the brain tissue was fixed. The total rearing period was 18 weeks. The relationship between homocysteine and stroke was described by plotting survival curves. The location and quantity of cerebral hemorrhage were determined through histopathological staining. ResultsThe serum Hcy concentration of mice fed with Hcy for 6 weeks increased to 23.07 mol/L, and the blood pressure ranged from 170 to 180 mmHg. The number of deaths due to cerebral hemorrhage was 10 in both the AngII + L - NAME + Hcy group and the AngII + L - NAME group. The p - value of the survival curves between the two groups was 0.162, indicating no statistically significant difference. ConclusionThe results demonstrated that elevated homocysteine levels did not influence the incidence of intracerebral hemorrhage in hypertensive mice. Hyperhomocysteinemia does not increase the risk of intracerebral hemorrhage in hypertensive mice