Response surface methodology for melanin nanoparticle production optimization from producer strain Pseudomonas stutzeri BTCZ305 with invitro anti-inflammatory and wound healing potential

Abstract

Culture conditions were optimized for the production of melanin nanoparticle by the bacterial strain Pseudomonas stutzeri BTCZ 305. Response surface methodology was employed for determining the most significant fermentation conditions using variables including, pH, temperature and L-tyrosine concentration identified through one-factor-at-a time approach. Box-behnken design consisting of 17 different combinations of all these factors were performed. Using this methodology, a quadratic regression model was built and the optimal combinations of media constituents for maximum melanin production 1192.27 g/mL were determined as temperature (32.5 {degrees}C), pH (8.5) and L-tyrosine concentration (7 g/L). Melanin productionwas obtained experimentally coincident with the predicted value and the model was proven to be adequate. The nanostructural distribution, its stability in colloidal suspension and particle size were also characterized with the help of TEM, particle size analysis and Zeta potential. The potent applicability of this molecule in anti-inflammation and wound healing was also elucidated. HighlightsO_LIMelanin nanoparticle synthesis by marine bacterial strain Pseudomonas stutzeri BTCZ 305 C_LIO_LIMedia optimization using one-factor-at-a-time method C_LIO_LIStatistical media optimization using Response surface methodology and Box-behnken design of experiment C_LIO_LINanoparticle characterization using TEM, particle size analysis and zeta potential. Its functional role in anti-inflammation and wound healing. C_LI